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KMID : 1094720150200050948
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 5 p.948 ~ p.955
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Characterization of hydroxyapatite-coated bacterial cellulose scaffold for bone tissue engineering
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Ahn Sung-Jun
Shin Young-Min
Kim Se-Eun
Jeong Sung-In
Jeong Jin-Oh
Park Jong-Seok
Gwon Hui-Jeong
Seo Da-Eun
Nho Young-Chang
Kang Seong-Soo
Kim Chong-Yeal
Huh Jung-Bo
Lim Youn-Mook
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Abstract
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The goal of this study was to develop a novel hydroxyapatite (HA) coated bacterial cellulose (BC) scaffold for bone tissue regeneration. HA-coated BC was prepared by immersing in 30 mL of 5× simulated body fluid at 37°C for 12 h. The resulting HA-coated BC scaffolds were characterized by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared (ATRFTIR) spectroscopy, and thermal gravimetric analysis (TGA). HA spherical globules were newly formed on the surface of the BC, and a fibrous network of BC scaffolds still maintained their dimensions for cell adhesion and proliferation. ATR-FTIR spectroscopy analysis showed bands assigned to specific signals for phosphate and carbonate ions from HA. HA-coated BC scaffolds of thermal gravimetric analysis presented residue of around 25%. The ability for bone regeneration of HA-coated BC scaffolds was evaluated using a rat calvarial defect model for 4 and 8 weeks. After implantation, both BC and HAcoated BC scaffolds showed new bone formation derived from existing bone, and found new bone even inside the scaffold. Furthermore, a new bone area was signigicantly increased in the HA-coated BC scaffolds compared with those from BC scaffolds, and bone-like materials were frequently found in HA-coated BC scaffolds. Therefore, the HA-coated BC scaffolds can be used as an effective tool for bone tissue regeneration.
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KEYWORD
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bacterial cellulose, radiation, hydroxyapatite, bone tissue engineering
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